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Sequence analysis of OsRBSq3 . ( A ) Proteins with high sequence similarity to OsRBSq3 were identified in Aegilops tauschii , Brachypodium distachyon , Hordeum vulgare , Lolium perenne , Oryza sativa ssp. japonica , Triticum aestivum , and Triticum Urartu . Multiple sequence alignments was performed using BioEdit (version 7.0; https://bioedit.software.informer.com/ ) ( B ) Phylogenetic analysis of OsRBSq3 and its homologs was conducted using the maximum likelihood method with 1,000 bootstrap replicates in MEGA (version 12; https://www.megasoftware.net/ ) ( C ) Predicted protein–protein interactions of OsRBSq3 were analyzed using the STRING database (version 12; https://string-db.org/ ), showing associations with 10 proteins: TRE_ORYSJ, PMM, A0A0P0VPT7, A0A0P0W356, A0A0P0WVQ1, A0A0P0XFZ1, AMY1.1, AMY1.2, AMY1.3, and AMY1.4.
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Sequence analysis of OsRBSq3 . ( A ) Proteins with high sequence similarity to OsRBSq3 were identified in Aegilops tauschii , Brachypodium distachyon , Hordeum vulgare , Lolium perenne , Oryza sativa ssp. japonica , Triticum aestivum , and Triticum Urartu . Multiple sequence alignments was performed using BioEdit (version 7.0; https://bioedit.software.informer.com/ ) ( B ) Phylogenetic analysis of OsRBSq3 and its homologs was conducted using the maximum likelihood method with 1,000 bootstrap replicates in MEGA (version 12; https://www.megasoftware.net/ ) ( C ) Predicted protein–protein interactions of OsRBSq3 were analyzed using the STRING database (version 12; https://string-db.org/ ), showing associations with 10 proteins: TRE_ORYSJ, PMM, A0A0P0VPT7, A0A0P0W356, A0A0P0WVQ1, A0A0P0XFZ1, AMY1.1, AMY1.2, AMY1.3, and AMY1.4.
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Sequence analysis of OsRBSq3 . ( A ) Proteins with high sequence similarity to OsRBSq3 were identified in Aegilops tauschii , Brachypodium distachyon , Hordeum vulgare , Lolium perenne , Oryza sativa ssp. japonica , Triticum aestivum , and Triticum Urartu . Multiple sequence alignments was performed using BioEdit (version 7.0; https://bioedit.software.informer.com/ ) ( B ) Phylogenetic analysis of OsRBSq3 and its homologs was conducted using the maximum likelihood method with 1,000 bootstrap replicates in MEGA (version 12; https://www.megasoftware.net/ ) ( C ) Predicted protein–protein interactions of OsRBSq3 were analyzed using the STRING database (version 12; https://string-db.org/ ), showing associations with 10 proteins: TRE_ORYSJ, PMM, A0A0P0VPT7, A0A0P0W356, A0A0P0WVQ1, A0A0P0XFZ1, AMY1.1, AMY1.2, AMY1.3, and AMY1.4.
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Sequence analysis of OsRBSq3 . ( A ) Proteins with high sequence similarity to OsRBSq3 were identified in Aegilops tauschii , Brachypodium distachyon , Hordeum vulgare , Lolium perenne , Oryza sativa ssp. japonica , Triticum aestivum , and Triticum Urartu . Multiple sequence alignments was performed using BioEdit (version 7.0; https://bioedit.software.informer.com/ ) ( B ) Phylogenetic analysis of OsRBSq3 and its homologs was conducted using the maximum likelihood method with 1,000 bootstrap replicates in MEGA (version 12; https://www.megasoftware.net/ ) ( C ) Predicted protein–protein interactions of OsRBSq3 were analyzed using the STRING database (version 12; https://string-db.org/ ), showing associations with 10 proteins: TRE_ORYSJ, PMM, A0A0P0VPT7, A0A0P0W356, A0A0P0WVQ1, A0A0P0XFZ1, AMY1.1, AMY1.2, AMY1.3, and AMY1.4.
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Sequence analysis of OsRBSq3 . ( A ) Proteins with high sequence similarity to OsRBSq3 were identified in Aegilops tauschii , Brachypodium distachyon , Hordeum vulgare , Lolium perenne , Oryza sativa ssp. japonica , Triticum aestivum , and Triticum Urartu . Multiple sequence alignments was performed using BioEdit (version 7.0; https://bioedit.software.informer.com/ ) ( B ) Phylogenetic analysis of OsRBSq3 and its homologs was conducted using the maximum likelihood method with 1,000 bootstrap replicates in MEGA (version 12; https://www.megasoftware.net/ ) ( C ) Predicted protein–protein interactions of OsRBSq3 were analyzed using the STRING database (version 12; https://string-db.org/ ), showing associations with 10 proteins: TRE_ORYSJ, PMM, A0A0P0VPT7, A0A0P0W356, A0A0P0WVQ1, A0A0P0XFZ1, AMY1.1, AMY1.2, AMY1.3, and AMY1.4.
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Sequence analysis of OsRBSq3 . ( A ) Proteins with high sequence similarity to OsRBSq3 were identified in Aegilops tauschii , Brachypodium distachyon , Hordeum vulgare , Lolium perenne , Oryza sativa ssp. japonica , Triticum aestivum , and Triticum Urartu . Multiple sequence alignments was performed using BioEdit (version 7.0; https://bioedit.software.informer.com/ ) ( B ) Phylogenetic analysis of OsRBSq3 and its homologs was conducted using the maximum likelihood method with 1,000 bootstrap replicates in MEGA (version 12; https://www.megasoftware.net/ ) ( C ) Predicted protein–protein interactions of OsRBSq3 were analyzed using the STRING database (version 12; https://string-db.org/ ), showing associations with 10 proteins: TRE_ORYSJ, PMM, A0A0P0VPT7, A0A0P0W356, A0A0P0WVQ1, A0A0P0XFZ1, AMY1.1, AMY1.2, AMY1.3, and AMY1.4.
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Image Search Results


Sequence analysis of OsRBSq3 . ( A ) Proteins with high sequence similarity to OsRBSq3 were identified in Aegilops tauschii , Brachypodium distachyon , Hordeum vulgare , Lolium perenne , Oryza sativa ssp. japonica , Triticum aestivum , and Triticum Urartu . Multiple sequence alignments was performed using BioEdit (version 7.0; https://bioedit.software.informer.com/ ) ( B ) Phylogenetic analysis of OsRBSq3 and its homologs was conducted using the maximum likelihood method with 1,000 bootstrap replicates in MEGA (version 12; https://www.megasoftware.net/ ) ( C ) Predicted protein–protein interactions of OsRBSq3 were analyzed using the STRING database (version 12; https://string-db.org/ ), showing associations with 10 proteins: TRE_ORYSJ, PMM, A0A0P0VPT7, A0A0P0W356, A0A0P0WVQ1, A0A0P0XFZ1, AMY1.1, AMY1.2, AMY1.3, and AMY1.4.

Journal: Scientific Reports

Article Title: Unveiling key genetic loci and candidate genes for brown spot disease resistance in rice based on QTL analysis

doi: 10.1038/s41598-025-33890-y

Figure Lengend Snippet: Sequence analysis of OsRBSq3 . ( A ) Proteins with high sequence similarity to OsRBSq3 were identified in Aegilops tauschii , Brachypodium distachyon , Hordeum vulgare , Lolium perenne , Oryza sativa ssp. japonica , Triticum aestivum , and Triticum Urartu . Multiple sequence alignments was performed using BioEdit (version 7.0; https://bioedit.software.informer.com/ ) ( B ) Phylogenetic analysis of OsRBSq3 and its homologs was conducted using the maximum likelihood method with 1,000 bootstrap replicates in MEGA (version 12; https://www.megasoftware.net/ ) ( C ) Predicted protein–protein interactions of OsRBSq3 were analyzed using the STRING database (version 12; https://string-db.org/ ), showing associations with 10 proteins: TRE_ORYSJ, PMM, A0A0P0VPT7, A0A0P0W356, A0A0P0WVQ1, A0A0P0XFZ1, AMY1.1, AMY1.2, AMY1.3, and AMY1.4.

Article Snippet: To identify homologous gene sequences, sequence similarity searches were conducted using NCBI BLAST, and multiple sequence alignments were performed using BioEdit version 7.0 ( https://bioedit.software.informer.com/ ) .

Techniques: Sequencing, Software, Protein-Protein interactions